Collins played 90 minutes in West Ham's 2-0 win against Wolves
James Collins' move to Stoke City has fallen through after the Welsh international failed to agree terms at the Britannia Stadium.
Stoke had a bid of about £3m - rising to a potential £5m - accepted by Premier League rivals West Ham United.
BBC Sport understands the 25-year-old defender cannot agree personal terms.
"We just couldn't come to an agreement," Collins' agent Cyrille Regis told BBC Sport. "So at the moment the deal is dead."
Stoke boss Pulis admitted on Tuesday that Collins was "one of a number of players I'm looking at" as he aims to strengthen his defensive options.
The Welsh defender was at the Britannia Stadium on Tuesday discussing personal terms but talks broke down.
Collins has spent four seasons at Upton Park, although injuries have limited him to only 49 league starts for the Hammers.
He played the full 90 minutes as Gianfranco Zola's side won their opening Premier League of the season 2-0 against promoted Wolves at Molineux on Saturday.
West Ham boss Zola insisted his club did not need to sell key players before the end of the August transfer window.
Zola was thought to be under pressure from CB Holding, the club's Icelandic owners, to cash in on the squad.
But Zola told the club's website on Tuesday: "We are looking to buy not sell players."
Meanwhile, Pulis has made it clear he wants Ryan Shawcross to remain at the Britannia Stadium.
Reports have linked the 21-year-old defender with a move to Manchester United, Liverpool or Tottenham Hotspur.
Pulis said: "Ryan's done fantastically well for us and we want to keep him at Stoke City. He's one of the younger players that we've got in the group and he's a big asset to us.
"This club is looking to push on and you don't do that by selling your best players."
Stoke, who won their opening game of the season against Burnley on Saturday, travel to Anfield on Wednesday to take on Liverpool.
E-mail this to a friend 
Printable version 
~RS~a~RS~Domestic~RS~q~RS~~RS~z~RS~28~RS~" height="1" width="2" alt="" /></p loading=)
Bookmark with:
What are these?