Separating dissolved solids – chromatography
Paper chromatographyChromatography is used to separate different substances dissolved in a liquid. is a method for separating dissolved substances from one another. It is often used when the dissolved substances are coloured, such as inks, food colourings and plant dyes. It works because some of the coloured substances dissolve in the solvent used better than others, so they travel further up the paper.
Image caption, A pencil line is drawn, and spots of ink or plant dye are placed on it. There is a container of solvent, such as water or ethanol.
Image caption, The paper is lowered into the solvent. The solvent travels up through the paper, taking some of the coloured substances with it.
Image caption, As the solvent continues to travel up the paper, the different coloured substances spread apart. In this example, the colours separated substances are yellow, red and black.
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A pure substance will only produce one spot on the chromatogram during paper chromatography. Two substances will be the same if they produce the same colour of spot, and their spots travel the same distance up the paper. In the example below, red, blue and yellow are three pure substances. The sample on the left is a mixture of all three.