Lincoln City's run of pre-season victories came to an end at Blundell Park as they lost 1-0 to Grimsby Town.
With the Imps resting several players, the game's only goal came after 29 minutes when Peter Bore intercepted Stuart Giddings' back-pass to score.
Coach Grant Brown told BBC Radio Lincolnshire: "It was a bit disjointed at times, but on the positive side a lot of players got a full run-out.
"We'll keep progressing with each game to where we want to be on 9 August."
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