Truro City dropped to third in the Southern League's Division One South and West after losing at Uxbridge.
The defeat was their first away from home all season after winning six out of their previous seven games.
Truro were 3-0 down after 48 minutes through Mark Nicholls, Dave Warner and Lee Tunnell before Andy Watkins got one back with 22 minutes to go.
But Nicholls and Dave Lawrence extended Uxbridge's lead, with Ian Stonebridge netting a last minute penalty for City.
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