Miguel Almiron became the first player to be sent off for covering his mouth in the World Cup but 10-man Paraguay bravely clung on to boost their last-32 hopes and eliminate Turkey.
Paraguay took the lead after just 64 seconds when midfielder Matias Galarza fired in from outside the area to score the fastest goal so far in this tournament and what turned out to be the match-winner.
Then former Newcastle United midfielder Almiron put his hand over his mouth in a confrontation with Turkey defender Mert Muldur during first-half stoppage time which led to a video assistant referee review and a sending off from Salvadoran referee Ivan Barton as part of a new rule introduced before the World Cup.
Both sides came into the game on the back of defeats with Paraguay thrashed by hosts USA 4-1 while Australia beat Turkey 2-0 - meaning there was an edge to the meeting with so much on the line, which led to a fiery contest.
Turkey dominated the first half but managed just one shot on target from 12 attempts while a deflected header from Muldur hit the bar and the post - before Almiron's dismissal then ensured it would be an even more difficult night for Paraguay.
Merih Demiral did test Orlando Gill after the break before substitute Deniz Gul wasted a great chance with a close-range header that was straight at the Paraguay goalkeeper.
Can Uzun forced Gill into a fine save late on before Gul somehow turned wide the rebound, although it was likely it would have been ruled out for offside to somewhat spare his blushes.
Turkey had 30 attempts on goal against the Socceroos in their first game and a further 32 here but could not find a way through against the 10 men in the second half and remain goalless in this tournament.
They cannot progress now after two defeats while Paraguay know if they beat Australia in their final Group D game, then they will be through to the knockout stages.
