Guiseley's reward for knocking holders Barrow out of the FA Trophy is a second round home tie against Blue Square Bet North rivals Stalybridge.
Boston United, who created a minor upset with a 1-0 first round win against York, will face Gloucester or Cirencester City.
Blue Square Bet Premier leaders AFC Wimbledon have been handed a Surrey derby with Woking.
The ties are scheduled to take place on Saturday, 15 January.
FA Trophy Second Round:
Gateshead v Hampton & Richmond Borough Boston United v Gloucester City AFC Wimbledon v Woking Droylsden v Ebbsfleet United Chasetown v Grimsby Town Alfreton Town v Cambridge United Ashford Town (M'sex) v Dartford Luton Town v Uxbridge AFC Telford United v Eastwood Town Darlington v Bath City Mansfield Town v Newport County Blyth Spartans AFC v Altrincham Dorchester Town v Eastbourne Borough Guiseley AFC v Stalybridge Celtic Eastleigh v Worcester City Salisbury City v Wrexham
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