Leaders Burton came from a goal down to earn a dramatic late victory.
Eastbourne made Burton pay for sloppy early finishing when Daniel Brown headed Matt Crabb's corner into the top corner from the edge of the box.
But the home side were pegged back when Martin Butler got on the end of Darren Stride's pass and found the bottom corner from 12 yards.
Burton then snatched victory when Greg Pearson headed home Mark Byrne's cross in the fifth minute of injury-time.
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Eastbourne Borough:
Hook, Baker, Jenkins (Harding 90), Austin, Pullan, Lovett, Armstrong, Brown, Matt Crabb, Jeffery (Nathan Crabb 58), Gargan (Atkin 58). Subs Not Used: Smart, Wormull.
Booked: Baker, Jenkins.
Goals: Brown 25.
Burton Albion:
Poole, James, Buxton, Webster, Simpson, McGrath, Gilroy, Stride (Byrne 73), Corbett, Harrad (Pearson 86), Morris (Butler 61). Subs Not Used: Deeney, Austin.
Booked: Webster.
Goals: Butler 63, Pearson 90.
Att: 1,521
Ref: K Johnson (Bristol)
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