Phil Bolland's late goal earned Cambridge United all three points as they continued their promotion push.
Bolland rose above the Diamonds defence with a minute remaining and headed home Courtney Pitt's left-wing cross.
The visitors had taken an early lead when Chris Holroy converted from the spot after Chris Hope had clumsily brought down Danny Crow in the area.
Just before the break Rushden equalised when Sam Smith found the net from 18-yards before Bolland's late winner.
Rushden & Diamonds: Roberts, Corcoran, Osano, Hope, Robinson, Tomlin, Beecroft, Wolleaston, Kelly (Broadbent 90), Cousins (McGuinness 84), Smith. Subs Not Used: Day, Hilliard, Cox.
Booked: Robinson, Corcoran.
Goals: Smith 42.
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Booked: Crow.
Goals: Holroyd 22 pen, Bolland 89.
Att: 2,058
Ref: M Brown (England).
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