Mansfield came from behind to earn victory and end Eastbourne's four-game winning streak.
Andy Atkin headed Dan Smith's right-wing cross beyond Mansfield goalkeeper Alan Marriott.
The visitors levelled when Captain Jonathan D'Laryea surged into the box and met Mathew Somner's cross from six yards out.
Scott Garner rose to head in Michael Blackwood's cross from the left wing to claim the win for the Stags.
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Goals: Atkin 7.
Booked: Silk.
Goals: D'Laryea 45, Garner 79.
Att: 1,420
Ref: J Farries (Oxfordshire).
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