Goals from Phil Trainer and James Constable handed Oxford a much-needed victory and ended Burton's five game unbeaten run.
The hosts opened the scoring after just eight minutes when Trainer converted Lewis Haldane's cross from 12 yards.
Burton's Lee Morris missed two chances to level before Ryan Austin fouled Constable in the box and the fit-again top scorer converted from the spot.
Aaron Webster's 20-yard free kick on 89 minutes was of little consolation.
Oxford Utd: Turley, Clarke, Quinn, Foster, Carruthers, Haldane (Taylor 83), Murray, Trainer, Deering, Guy (Odubade 73), Constable. Subs Not Used: Hinchliffe, Day, Hutchinson.
Booked: Quinn, Foster.
Goals: Trainer 8, Constable 50 pen.
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Booked: Simpson, Goodfellow.
Goals: Webster 89.
Att: 4,494
Ref: P Tierney (Wigan).
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