Eddie Ancalet struck early in the second half to earn victory for Oxford.
York almost took the lead early on but Leo Fortune-West's left-foot volley flew over the crossbar.
Oxford keeper Billy Turley saved from David McGurk after he headed goalwards from a corner and then a long-range strike from Martyn Woolford.
Ancalet struck with a six-yard effort after the break but Emmanuel Panther almost equalised with a volley that was blocked by Matt Day.
York: Mimms, McWilliams, McGurk, Parslow, Purkiss, Beadle (Boyes 88), Panther, Fortune-West, Woolford, Sodje, Brodie (Shepherd 79). Subs Not Used: Robinson, McDonald, Lloyd.
Oxford Utd: Turley, Day, Clarke, Quinn, Anaclet, Trainer, Murray, Hand, Howard, Odubade (Richards 86), Green (McAllister 63). Subs Not Used: Pettefer, Hutchinson, Rose.
Goals: Anaclet 48.
Att: 1,808
Ref: D Mohareb (Cheshire).
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