Holders: Etoile du Sahel (TUN)

Second Round:

21-23/03/08: Enyimba (NGR) 4-0 Simba (TAN)

21-23/03/08: Asko Kara (TOG) 2-0 Club Africain (TUN)

21-23/03/08: Cotonsport (CMR) 5-0 Gombe Utd (NGR)

21-23/03/08: Asec Mimosas (CIV) 1-1 AS Kaloum (GUI)

21-23/03/08: Olympic Khouribga (MOR) 2-0 Entente Setif (ALG)

21-23/03/08: Sporting Club Praia (CPV) 2-1 InterClube (ANG)

21-23/03/08: Zamalek (EGY) 2-0 Africa Sports (CIV)

21-23/03/08: Etoile du Sahel (TUN) 5-0 AS Douanes (SEN)

21-23/03/08: Dynamos (ZIM) 3-0 Costa do Sol (MOZ)

21-23/03/08: JS Kabylie (ALG) 3-0 AshantiGold (GHA)

21-23/03/08: Cotonsport (CMR) 5-0 Gombe Utd (NGR)

21-23/03/08: TP Mazembe (DRC) 1-1 FC 105 Libreville (GAB)

21-23/03/08: Platinum Stars (RSA) 2-0 Stade Tamponnaise (REU)

21-23/03/08: Mamelodi Sundowns (RSA) 3-0 Curepipe Starlight (MRI)

Al Ahly (EGY) receive a bye to the next round after Al Tahrir (ERI) pulled out.

First round:

15-17/02/08: ASC SNIM (MTN) 1-5 Entente Setif (ALG)
01-02/03/08: Entente Setif 2-0 ASC SNIM
Entente Setif win 7-1 on aggregate

15-17/02/08: Sporting Bissau (GNB) 0-2 Olympique Khouribga (MOR)
01-02/03/08: Olympique Khouribga 2-0 Sporting Bissau
Olympique Khouribga win 4-0 on aggregate

15-17/02/08: Invincible XI (LIB) v AS Kaloum (GUI)
AS Kaloum advance after Invincible XI withdraw

15-17/02/08: Renacimiento (GEQ) 1-2 InterClube (ANG)
01-02/03/08: InterClube 2-1 Renacimiento
InterClube win 4-2 on aggregate

15-17/02/08: FAR Rabat (MOR) 3-0 Sporting Praia (CAP)
01-02/03/08: Sporting Praia 3-0 FAR Rabat
3-3 on aggregate,Sporting Praia win 5-4 on penalties

15-17/02/08: Tonnerre FC (BEN) 0-0 Africa Sports (CIV)
01-02/03/08: Africa Sports 3-0 Tonnerre FC
Africa Sports win 3-0 on aggregate

15-17/02/08: APR FC (RWA) 1-2 Zamalek (EGY)
01-02/03/08: Zamalek 2-0 APR FC
Zamalek win 4-1 on aggregate

15-17/02/08: AS Douanes (SEN) 0-0 CF Ouagadougou (BFA)
01-02/03/08: CF Ouagadougou 2-3 AS Douanes
AS Douanes win 3-2 on aggregate

15-17/02/08: Costa do Sol (MOZ) 2-0 Ajesaia (MAD)
01-02/03/08: Ajesaia 1-0 Costa do Sol
Costa do Sol win 2-1 on aggregate

15-17/02/08: Royal Leopards (SWZ) 0-1 Dynamos (ZIM)
01-02/03/08: Dynamos 2-0 Royal Leopards
Dynamos win 3-0 on aggregate

15-17/02/08: Sahel SC (NIG) 1-0 AshantiGold (GHA)
01-02/03/08: AshantiGold 6-1 Sahel SC
AshantiGold win 6-2 on aggregate

15-17/02/08: DC Motema Pembe (DRC) 2-0 Gombe United (NGR)
01-02/03/08: Gombe United 2-0 DC Motema Pembe
2-2 on aggregate,Gombe United win 4-1 on penalties

15-17/02/08: Vital'O (BUR) 0-1 Cotonsport (CMR)
01-02/03/08: Cotonsport 1-0 Vital'O
Cotonsport win 2-0 on aggregate

15-17/02/08: FC105 Libreville (GAB) 3-0 Hearts of Oak (GHA)
01-02/03/08: Hearts of Oak 3-1 FC105 Libreville
FC105 Libreville win 4-3 on aggregate

15-17/02/08: Renaissance (CHA) v TP Mazembe (DRC)
TP Mazembe advance due Caf ban on clubs from Chad

15-17/02/08: Stade Malien (MLI) 1-2 Primeiro Agosto (ANG)
01-02/03/08: Primeiro Agosto 0-0 Stade Malien
Primeiro Agosto win 2-1 on aggregate

15-17/02/08: ASC Saloum (SEN) 1-2 Club Africain (TUN)
01-02/03/08: Club Africain 1-0 ASC Saloum
Club Africain win 3-1 on aggregate

15-17/02/08: Asko Kara (TOG) 3-1 Union Douala (CMR)
01-02/03/08: Union Douala 0-1 Asko Kara
Asko Kara win 4-1 on aggregate

15-17/02/08: Simba SC (TAN) 3-0 Awassa City (ETH)
01-02/03/08: Awassa City 1-1 Simba SC
Simba SC win 4-1 on aggregate

15-17/02/08: Enyimba (NGR) 4-1 Diables Noirs (CGO)
01-02/03/08: Diables Noirs 2-3 Enyimba
Enyimba win 7-3 on aggregate

15-17/02/08: Tusker FC (KEN) v Al Tahrir (ERI)
Al Tahrir advance due to Caf ban clubs from Kenya

15-17/02/08: US Tamponnaise (REU) 3-1 St Michel United (SEY)
01-02/03/08: St Michel United 0-1 US Tamponnaise
US Tamponnaise win 4-1 on aggregate

15-17/02/08: Platinum Stars (RSA) 4-0 Correction Services (LES)
01-02/03/08: Correction Services 1-0 Platinum Stars
Platinum Stars win 4-1 on aggregate

15-17/02/08: Curepipe Starlight (MRI) 2-0 Coin Nord Mitsamiouli (COM)
01-02/03/08: Coin Nord Mitsamiouli 1-0Curepipe Starlight
Curepipe Starlight win 2-1 on aggregate

15-17/02/08: Miembeni SC (ZNZ) 0-1 Mamelodi Sundowns (RSA)
01-02/03/08: Mamelodi Sundowns 5-0 Miembeni SC
Mamelodi Sundowns win 5-0 on aggregate

15-17/02/08: URA (UGA) 0-2 ZESCO United (ZAM)
01-02/03/08: ZESCO United 0-0 URA
ZESCO United win 2-0 on aggregate