"This is a fantastic achievement from all players in the Barclays Premier League, and I am proud to have helped raise such a significant amount of money for this year's Sport Relief campaign." said Flamini.
Professional match analysts Prozone and AMISCO monitored the combined mileage of all Barclays Premier League players in matches played between 15-17 March and calculated that players ran a total of 1454.3 miles (2340.5 km) over the 10 games.
Barclays will roundup its Sport Relief donation to a total of �55,000 - the exact figure raised by players was �43,629.
The Sainsbury's Sport Relief Mile events on Sunday March 16 saw over 100,000 people take to the streets across the UK to run a Mile and raise money.
By the end of the Sport Relief weekend an impressive �20,747,375 had been raised and that figure should rise further when sponsorship money is paid.
All the money raised is spent by Comic Relief to help vulnerable people living incredibly tough lives both at home in the UK and in the world's poorest countries.
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