The experiment
This is a common experiment used to model the digestive system and will help you understand how to work scientifically.
Try the experiment in the lab
Aim
To use Visking tubing to model the digestive system and show what substances can pass through its lining.
Method
Set up two sets of the apparatusPieces of equipment. as in the diagram.
Put 1cm³ of amylase enzyme into tube 1 but not tube 2.
Leave at room temperature for five minutes.
Test for starch (using iodine) and glucose (using Benedict’s reagent) inside and outside both tubes.
Variables
Independent variable: the presence or absence of amylase.
Dependent variable: the presence or absence of glucose inside and outside the tubing.
Controlled variables: the quantities of the solutions used, the time taken before testing and the temperature of the water.
Risks
What are the risks to safety in this experiment?
Care must be taken with glassware, iodine solution and Benedict’s solution.
Results
Expected results Tube 1 (with amylase)
| Inside | Outside | |
|---|---|---|
| Starch present | Yes | No |
| Glucose present | Yes | Yes |
Expected results Tube 2 (without amylase)
| Inside | Outside | |
|---|---|---|
| Starch present | Yes | No |
| Glucose present | No | No |
What the results mean
There is no starch outside the Visking tubing. It is too large to pass through the tubing.
Glucose is present in the solution containing amylase (tube 1), but not in the solution that doesn't contain amylase (tube 2). Amylase breaks down starch into glucose.
There is glucose outside the Visking tubing in tube 1 as glucose is small enough to pass through the tubing.
Evaluation
At the end of an investigation, it is important to evaluateTo consider the quality of data and suggest improvements to a method. the results and the method. After this is done, a judgement is made about how reliable the conclusion is.
Try an online experiment
Use Benedict's reagent in another experiment in Atomic Labs. Go to the Biology lab and try the Testing for sugar experiment.
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