What is an aseptic technique?
To safely grow uncontaminated bacterial colonies an Name given to the laboratory procedures carried out to prevent the contamination of pure cultures of microorganisms. is used:
Turn on a Bunsen burner to kill airborne microorganisms.
Pass a metal loop through the flame to sterilise it.
Allow the loop to cool to avoid killing bacteria to be grown.
Flame the neck of the In microbiology, a colony of microbes, typically on an agar plate. bottle to sterilise it after removing its lid.
Dip the loop into the bacterial culture to To introduce bacterial cells. it.
Partially lift the lid of the A clear glass or plastic dish, used to grow living cells from organisms so they can be studied. to minimise contamination.
Glide the loop over the agar surface to spread bacteria.
Pass the loop through the flame again to sterilise it.
Tape the lid onto the Petri dish.
Incubate at 25°C – below body temperature to ensure pathogenic microorganisms do not grow. Incubate upside down to avoid condensation dripping on the bacteria.
Clean work surfaces and hands using disinfectant/alcohol to kill bacteria.
Dispose of cultures by An autoclave is a machine that uses steam under pressure to kill harmful bacteria, viruses, fungi, and spores on items that are placed inside a pressure vessel. to ensure safety.
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