Mixing particles
If a crystal of a coloured soluble chemical, eg potassium manganate(VII), is placed in water, the particles spread out and mix with the water particles.
When the potassium manganate(VII) has dissolveWhen a substance breaks up and mixes completely with a solvent to produce a solution. it becomes the soluteThe dissolved substance in a solution..
The water is the solventThe liquid in which the solute dissolves to form a solution.. The mixture that results is the solutionMixture formed by a solute and a solvent..
The particles have diffused from a region of high concentrationThe concentration of a solution tells us how much of a substance is dissolved in water. The higher the concentration, the more particles of the substance are present. in the crystal to a low concentration in the water. This difference in concentration is called a concentration gradientThe difference in the concentration of a chemical across a membrane.. Particles will move down a concentration gradient, from a high concentration to a low concentration.
As well as diffusion occurring between different regions, it also occurs across membranes, between the outside and inside of cells.
The rate of diffusion
The rate of diffusion can be affected by several factors:
| Factor | Effect on diffusion |
| Concentration gradient | The greater the difference in concentration, the quicker the rate of diffusion. |
| Temperature | The higher the temperature, the more kinetic energy the particles will have, so they will move and mix more quickly. |
| Surface area of the cell membrane separating the different regions | The greater the surface area, the faster the rate of diffusion. |
| Factor | Concentration gradient |
|---|---|
| Effect on diffusion | The greater the difference in concentration, the quicker the rate of diffusion. |
| Factor | Temperature |
|---|---|
| Effect on diffusion | The higher the temperature, the more kinetic energy the particles will have, so they will move and mix more quickly. |
| Factor | Surface area of the cell membrane separating the different regions |
|---|---|
| Effect on diffusion | The greater the surface area, the faster the rate of diffusion. |
Diffusion, surface area and volume
In a bacteriaSingle-celled microorganisms, some of which are pathogenic in humans, animals and plants. Singular is bacterium., substances diffuse into and out of the bacterial cell across its surface. Once inside, because of the bacterium’s size, substances will need to diffuse 1 μm or less to where they are needed, for instance oxygen for aerobic respiration.
For simple multicellularHaving more than one cell.organismLiving entity, eg animals, plants or microorganisms. such as small plants like mosses, substances diffuse into the leaves and simple roots over their surface. Again, once inside the plant, they don’t need to move far.
Substances move into and around the moss plants by diffusion and osmosisThe movement of water molecules across a selectively permeable membrane from a region of higher water concentration to a region of lower water concentration..
Simple organisms therefore take in substances all over their body surface. The amount of each substance they need is determined by their volume. As organisms increase in size, their surface area does not increase at the same rate as their volume. This sets a size limit on organisms that can only use their body surface for taking in substances. Large organisms have a large volume but relatively small surface area. They have a small surface area:volume ratio and so need to evolve additional exchange surfaces to take in the materials they need.
The surface area to volume ratio of a puppy is several times greater than that of an adult dog.
Question
Suggest why puppies are more at risk of losing body heat than adult dogs.
Dogs lose heat over their body surface. Puppies have a larger surface area to volume ratio than adult dogs, so will lose heat more readily.
Osmosis
Osmosis is the diffusionThe movement of molecules from an area of higher concentration to an area of lower concentration. of water molecules, from a region where the water molecules are in higher concentration, to a region where they are in lower concentration, through a partially permeableAlso called semi-permeable. A partially permeable membrane allows water and other small molecules to pass through, but not larger molecules such as starch. membrane.
A dilute solutionMixture formed by a solute and a solvent. contains a high concentration of water molecules while a concentrated solution contains a low concentration of water molecules.
The image shows an example of osmosis showing the direction of movement of water between two different concentrations of sugar solutions:
Water molecules move from solution one to solution two.
When the concentration of water is the same on both sides of the membrane, the movement of water molecules will be the same in both directions. There will be no netThe overall effect of something when all additions and deductions are calculated. movement of water molecules. In theory, the level of solution two will rise, but this will be opposed by gravity and will be dependent on the width of the container.
Similar observations will be made with solutions containing different soluteThe dissolved substance in a solution., for instance, salt instead of sugar.
Osmosis across living cells
Cells contain diluteA dilute solution is one with a low concentration of solute. solutions of ionElectrically charged particle, formed when an atom or molecule gains or loses electrons., sugarA simple carbohydrate that is sweet to the taste. and amino acidThe building blocks that make up a protein molecule..
The cell membraneA selectively permeable membrane surrounding the cell and controlling the entry and exit of materials. is partially permeable.
Water will move into and out of cells by osmosis.
Plant cells
Isolated plant cells placed in a dilute solution or water will take in water by osmosis. If the soil is wet or moist, root hair cellA specialised cell that increases the surface area of the root epidermis to improve the uptake of water and minerals. will also take up water by osmosis. Leaf cells of land plants, unless it is raining or the humidityThe amount of water vapour in the atmosphere measured as a percentage. is high, will have a tendency to lose water.
Plant cells have a strong celluloseA carbohydrate. It forms the cell wall in plant cells.cell wallOuter structure which provides support and prevents the cell from bursting from the uptake of water by osmosis. Plant, fungal and bacterial cell walls have different structures and chemical compositions. outside the cell membrane. The cell wall is fully permeable to all molecules and supports the cell and stops it bursting when it gains water by osmosis.
If plant cells are placed in solutions of increasing solute concentration:
Pure water
In pure water, the cell contents - the cytoplasmThe living substance inside a cell (not including the nucleus). and vacuoleA space within the cytoplasm of plant cells that contains cell sap. – push against the cell wall and the cell becomes turgidEnlarged and swollen with water. Having turgor. Description of a plant cell in which the vacuole has swollen due to water gain by osmosis..
Fully turgid cells support the leaves and the stems of non-woody plants.
Concentrated solutions
In a more concentrated solution, the cell contents lose water by osmosis. They shrink and pull away from the cell wall. The cell becomes flaccidLacking turgor. Lacking in stiffness or strength. Soft and floppy.. It is becoming plasmolysedDescription of a plant cell in which the vacuole has shrunk and the membrane has pulled away from the wall due to water loss by osmosis..
Highly concentrated solution
In a very concentrated solution, the cell undergoes full plasmolysis as the cells lose more water.
Plants would be exposed to higher concentrations of soluteThe dissolved substance in a solution. if there was less water in the soil - for instance, if plants were not watered, or plants in drought conditions. Plant cells would then lose water by osmosisThe movement of water molecules across a selectively permeable membrane from a region of higher water concentration to a region of lower water concentration..
aquaticWithin water. , freshwater plants placed in the sea, or a seaweed in a rock pool where the water evaporated in the Sun, would also lose water by osmosis.
Animal cells
Animal cells also take in and lose water by osmosis. They do not have a cell wall, so will change size and shape when put into solutions that are at a different concentration to the cell contents.
For example, red blood cells:
In animals, the concentration of body fluids - blood plasmaThe liquid part of the blood containing useful things like glucose, amino acids, minerals, vitamins (nutrients) and hormones, as well as waste materials such as urea. and tissue fluidFluid which is derived from blood plasma that passes through the walls of capillaries. - must be kept within strict limits. If cells lose or gain too much water by osmosis, they do not function efficiently.