 Keothavong is ranked 151 in the world |
Andy Murray and Tim Henman will be Britain's only representatives in the singles events at the US Open. Anne Keothavong was the last player with a chance of joining them in the main draw, having reached the third round of qualifying in New York.
But the British number two retired from her match against Petra Cetkovska of the Czech Republic at 6-3 3-0 down.
Naomi Cavaday and Jamie Baker also lost matches for places in the main draw, to Sandra Kloesel and Pablo Cuevas.
Richard Bloomfield lost in the second round, while Alex Bogdanovic lost his opening match.
Melanie South and Elena Baltacha also failed to make it past the first round.
The US Open, the final Grand Slam of the year, begins at Flushing Meadows on Monday, 27 August.
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