Jelena Dokic was beaten in straight sets by 19-year-old American qualifier Shenay Perry in the second round of the DFS Classic in Birmingham. Dokic destroyed a racket in frustration in the second set as she fell to a 6-4 7-6 (7/4) defeat.
Defending champion Magdalena Maleeva recovered from a set and 0-3 down to beat German Anca Barna 3-6 6-4 6-3.
But British interest ended when Anne Keothavong was beaten 6-3 4-6 6-4 by 16th seed Maria Sanchez Lorenzo.
Keothavong displayed her full array of shots in the second set to level the match and then fought back from 5-2 down in the decider.
But an over-rule while trailing 0-15 in the final game appeared to affect her concentration.
Dokic admitted she had not played well but was at a loss to explain what the problem was.
"At times I can play well but my game is up and down at the moment," she said.
"I've not played well this year and my confidence is very low."
Slovak Daniela Hantuchova was forced to retire through illness against Tatiana Golovin when she was trailing 0-6 1-3.