Accrington will release Michael Welch, Mauro Almeida and Sean Doherty at the end of their contracts in the summer. Centre-back Welch, 23, joined from Macclesfield in 2005 and has been a regular in the last two seasons.
Fellow defender Almeida, 25, was signed on a short-term deal but made just five appearances for the club and has not featured since March.
Midfielder Doherty, 21, joined from Port Vale last summer and had a loan spell at Southport earlier this season.