Manchester United and Tottenham reached last year's Carling Cup final
The Carling Cup second round draw will take place on Wednesday evening, after the last three first-round games have been completed.
The 35 first-round winners are joined by Middlesbrough and Newcastle who had first-round byes, and the 13 Premier League clubs not playing in Europe.
The 25 highest-ranked clubs, based on last season's league places, have been seeded and so cannot face each other.
However, there is no geographical north/south split in this round.
Clubs playing in European competitions - Manchester United, Liverpool, Chelsea, Arsenal, Everton, Aston Villa and Fulham - will enter the competition in round three.
Seeded teams for second round draw:
Birmingham, Blackburn, Bolton, Bristol City, Burnley, Cardiff, Hull, Ipswich, Manchester City, Middlesbrough, Newcastle, Portsmouth, Preston, QPR, Reading, Sheffield Wednesday, Stoke, Sunderland, Swansea, Tottenham, Watford, West Bromwich Albion, West Ham, Wigan, Wolves.
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