Motherwell striker Ross McCormack is one of five uncapped players in George Burley's Scotland squad to face the Czech Republic in Prague on 30 May.
Rangers pair Kevin Thomson and Steven Whittaker, Hearts defender Christophe Berra and West Brom midfielder James Morrison could win their first caps.
But Celtic midfielder Scott Brown has been left out as he requires a small operation at the end of the season.
Captain Barry Ferguson is being rested because of a troublesome ankle injury.
"Barry has been struggling for the last few months with an ankle problem and, because of the amount of games he's played, I thought it was important to allow that to settle down," said Burley.
Rangers team-mates Allan McGregor, Lee McCulloch and veteran David Weir are also not included in the squad for the friendly.
We have 16 players who are 26 and under, so it is a squad who are progressing in their careers
Scotland manager George Burley
McGregor and McCulloch are currently out of action through injury.
Burley said of his selection: "I've got a youthful squad, there are five uncapped players and they will get a feel for it.
"We have 16 players who are 26 and under, so it is a squad who are progressing in their careers and who are still improving. We are looking forward to the next few years.
"It's a game at the end of the season and we are looking for young, fresh players with something to prove."
McCormack has impressed as Motherwell chase third place in the Scottish Premier League and a Uefa Cup place.
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