Scunthorpe sealed the final League One play-off place at the expense of Tranmere after captain Cliff Byrne snatched a dramatic late goal.
Tranmere had seemed set for a play-off place when Craig Curran headed home Ian Moore's cross at the far post.
But Rovers were reduced to 10 men when defender Gareth Edds was sent off for a second bookable offence
And Scunthorpe finally struck with just two minutes to go as Byrne headed home a free-kick from Grant McCann.
Scunthorpe: Lillis, Byrne, Mirfin, Pearce (Trotter 81), Mills (Wright 46), Sparrow (Thompson 64), Togwell, McCann, Woolford, Hayes, Hooper. Subs Not Used: Crosby, Slocombe.
Booked: McCann.
Goals: Byrne 88.
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Tranmere:
Coyne, Edds, Chorley, Shotton, Andy Taylor, Sonko, Kay (Ash Taylor 90), Jennings, Barnett (Cresswell 76), Curran, Moore. Subs Not Used: Achterberg, Gornell, Burns.
Sent Off: Edds (87).
Booked: Edds, Jennings.
Goals: Curran 39.
Att: 8,029
Ref: Steve Tanner (Somerset).
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