Peterborough missed the chance to close the gap on League One's automatic promotion places after they were held to a draw at Prenton Park.
Tranmere took the lead when Charlie Barnett shot into the top corner.
The visitors equalised before the break when Craig Mackail-Smith fired home after Aaron Creswell's clearance rebounded off United's Aaron McLean.
Bas Savage thought he had won it near the end for Tranmere but his strike was ruled out for offside.
Tranmere: Achterberg, Shotton, Chorley, Kay, Cresswell, Sonko (Shuker 63), Jennings, Edds, Barnett, Savage, Moore. Subs not used: Kennedy, Gornell, Waterfall, Henry.
Goal: Barnett 16.
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Peterborough: Lewis, Martin, Zakuani, Chester, Lee, Whelpdale (Frecklington 34), Keates, Coutts, Boyd, Mclean, Mackail-Smith. Subs not used: Torres, Green, Wright, Blanchett.
Booked: Zakuani, Coutts
Goal: Mackail-Smith 37
Att: 4,862
Ref: P Gibbs (West Midlands)
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