Andy Booth's eighth goal of the season was enough to give Huddersfield victory and end Tranmere's play-off hopes.
Booth, whose contract expires at the end of the season, tapped home after Michael Collins' toe-poke was parried by Rovers goalkeeper Danny Coyne.
Chris Shuker went closest in the first half for the visitors but was foiled by a fine save from Matt Glennon.
Booth almost grabbed a second late on for the hosts but he could not convert after a goalmouth scramble.
Huddersfield: Glennon, Nathan Clarke, Page, Mirfin, Holdsworth, Collins, Skarz, Tom Clarke, Williams, Booth (Beckett 81), Kamara (Schofield 52).Subs Not Used: Smithies, Hardy, Harris.
Booked: Nathan Clarke.
Goals: Booth 61.
Tranmere: Coyne, Stockdale, Goodison, Kay, Taylor, Shuker, Jennings, Chorley, Sherriff (Mullin 76), Moore (Curran 76), Greenacre.Subs Not Used: Achterberg, Jones, Gornell.
Att: 8,315
Ref: Steve Bratt (W Midlands).
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